Puszkin S, Schook W, Maimon J, Puszkin E
Biochim Biophys Acta. 1977 Sep 27;494(1):144-9. doi: 10.1016/0005-2795(77)90142-8.
Immunological identification of an antigen resolved from a protein complex by sodium dodecyl sulfate polyacrylamide gel electrophoresis has been attained. The identification is based on the formation of immunoprecipitin lines after the antigen diffuses laterally from acrylamide gel transverse slices into a surrounding agarose gel. This technique was designed for study of contractile and regulatory protein complexes of non-muscle cells where the scarcity of tissue precludes easy purification or high yield of muscle-like proteins. It complements double-gel immunodiffusion or immunoelectrophoresis and its use may be extended to other protein complexes.
通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳从蛋白质复合物中解析出的抗原已实现免疫鉴定。该鉴定基于抗原从丙烯酰胺凝胶横向切片侧向扩散到周围琼脂糖凝胶后免疫沉淀线的形成。这项技术是为研究非肌肉细胞的收缩和调节蛋白复合物而设计的，在这些细胞中，组织稀缺使得难以轻松纯化或高产肌肉样蛋白。它补充了双凝胶免疫扩散或免疫电泳，其应用可能扩展到其他蛋白质复合物。
