Robison S H, Cantoni O, Costa M
Carcinogenesis. 1982;3(6):657-62. doi: 10.1093/carcin/3.6.657.
The effect of various metal compounds on the DNA of Chinese hamster ovary (CHO) cells was studied. Both NiCl2 and crystalline NiS caused DNA strand breaks in cultured CHO cells, whereas amorphous NiS did not. Strand breaks were quantitated by determining the number of average molecular weight of DNA following treatment with the metal compounds. Exposure of cells to crystalline NiS, CoS, CdS, AgS, CuS and Ni3S2 at 10 micrograms/ml for 24 h also induced DNA strand breaks. Similar exposure to activated charcoal, which was also actively phagocytosed, failed to cause any effect on the DNA of CHO cells. In the case of NiCl2 and NiS the effect was shown to be both time and dose dependent. Other soluble metal compounds such as HgCl2, CaCrO4, and CdCl2 also decreased the molecular weight of DNA while MnCl2, ZnCl2 and FeCl2 caused no significantly detectable change in DNA molecular weight. These effects, which occur at low metal concentrations suggest that nickel and other metals which cause cellular transformation have a very selective and specific effect upon DNA.
研究了各种金属化合物对中国仓鼠卵巢（CHO）细胞DNA的影响。NiCl₂和结晶态NiS均能导致培养的CHO细胞DNA链断裂，而非晶态NiS则不会。通过测定金属化合物处理后DNA平均分子量的数量来对链断裂进行定量。将细胞暴露于浓度为10微克/毫升的结晶态NiS、CoS、CdS、AgS、CuS和Ni₃S₂中24小时，也会诱导DNA链断裂。类似地，将细胞暴露于同样能被积极吞噬的活性炭中，对CHO细胞的DNA没有任何影响。就NiCl₂和NiS而言，其影响表现出时间和剂量依赖性。其他可溶性金属化合物，如HgCl₂、CaCrO₄和CdCl₂也会降低DNA的分子量，而MnCl₂、ZnCl₂和FeCl₂对DNA分子量没有明显可检测到的变化。这些在低金属浓度下出现的影响表明，导致细胞转化的镍和其他金属对DNA具有非常选择性和特异性的作用。
