O'Connor R, Elliott W H, May B K
J Bacteriol. 1978 Oct;136(1):24-34. doi: 10.1128/jb.136.1.24-34.1978.
Late-log-phase cells of Bacillus amyloliquefaciens have the unusual capacity to produce extracellular protease for over 60 min in the presence of rifampin or actinomycin D at levels which strongly inhibit incorporation of amino acids into cellular protein. If cells are incubated in the presence of high levels of amino acids for 75 min this capacity is exhausted, but it is retained if the incubation is carried out in low levels of amino acids. Transfer of exhausted cells from high to low concentrations of amino acids results in a progressive recovery of the capacity for rifampin-actinomycin-insensitive protease production. The results seem best explained on the basis of the accumulation of a reserve pool of mRNA for extracellular protease. Measurement of the apparent mRNA pool size over 12 h shows a cyclical rise and fall, and these changes correlate with a periodic variation of the rate of protease production. A working hypothesis is presented to account for these observations in terms of a novel control situation over protease mRNA transcription.
解淀粉芽孢杆菌的对数生长后期细胞具有一种特殊能力，即在利福平或放线菌素D存在的情况下，能在60多分钟内持续产生胞外蛋白酶，而此时这些抗生素的浓度会强烈抑制氨基酸掺入细胞蛋白质。如果细胞在高浓度氨基酸存在下孵育75分钟，这种能力就会耗尽，但如果在低浓度氨基酸中进行孵育则会保留。将耗尽能力的细胞从高浓度氨基酸转移到低浓度氨基酸中，会使对利福平 - 放线菌素不敏感的蛋白酶产生能力逐渐恢复。这些结果似乎最好基于胞外蛋白酶mRNA储备池的积累来解释。对12小时内表观mRNA池大小的测量显示出周期性的上升和下降，并且这些变化与蛋白酶产生速率的周期性变化相关。本文提出了一个工作假说，以一种关于蛋白酶mRNA转录的新型控制情况来解释这些观察结果。
