解淀粉芽孢杆菌胞外酶的释放
Release of extracellular enzymes from Bacillus amyloliquefaciens.
作者信息
Gould A R, May B K, Elliott W H
出版信息
J Bacteriol. 1975 Apr;122(1):34-40. doi: 10.1128/jb.122.1.34-40.1975.
Abstract
Washed-cell suspensions of Bacillus amyloliquefaciens secrete significant amounts of the extracellular enzymes alpha-amylase and protease for about 15 min in the almost complete absence of protein synthesis. This apparently represents release of preformed enzyme en route to secretion. The release was independent of energy but was affected by temperature. Pulse-labeling experiments showed that newly synthesized enzyme molecules are either immediately released into the external medium or equilibrate with the preformed enzyme prior to eventual secretion. The results are compatible with a model of secretion whereby enzyme molecules emerging from the cell membrane become temporarily restricted by the cell wall so that a small pool of active enzyme accumulates in this region.
摘要
解淀粉芽孢杆菌的洗细胞悬液在几乎完全没有蛋白质合成的情况下，会在约15分钟内分泌大量细胞外酶α-淀粉酶和蛋白酶。这显然代表了在分泌途中预先形成的酶的释放。这种释放与能量无关，但受温度影响。脉冲标记实验表明，新合成的酶分子要么立即释放到外部介质中，要么在最终分泌之前与预先形成的酶达到平衡。这些结果与一种分泌模型相符，即从细胞膜出来的酶分子会暂时受到细胞壁的限制，从而在该区域积累一小部分活性酶。
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Evidence for an accumulation of messenger RNA specific for extracellular protease and its relevance to the mechanism of enzyme secretion in bacteria.细胞外蛋白酶特异性信使RNA积累的证据及其与细菌中酶分泌机制的相关性。
J Mol Biol. 1972 Jun 20;67(2):199-217. doi: 10.1016/0022-2836(72)90236-7.
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Biochim Biophys Acta. 1968 May 21;157(3):607-15. doi: 10.1016/0005-2787(68)90158-5.
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J Mol Biol. 1973 Jan 10;73(2):221-30. doi: 10.1016/0022-2836(73)90325-2.
7
Accumulation of messenger RNA for extracellular enzymes as a general phenomenon in Bacillus amyloiquefaciens.作为解淀粉芽孢杆菌中的一种普遍现象，细胞外酶信使核糖核酸的积累。
J Mol Biol. 1973 Jan 10;73(2):213-9. doi: 10.1016/0022-2836(73)90324-0.
8
A new ultrasensitive method for the determination of proteolytic activity.一种用于测定蛋白水解活性的新型超灵敏方法。
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