Hoober J K
J Bacteriol. 1978 Oct;136(1):359-68. doi: 10.1128/jb.136.1.359-368.1978.
Cells of a species of Arthrobacter were incubated in the light with methylene blue, a dye that sensitizes photooxidative reactions by the production of singlet oxygen. An early and major response by the cells to these conditions was stimulation of synthesis of a single cell-surface polypeptide, 21,000 daltons in mass. The rate of synthesis of this polypeptide reached a maximal level about 30 min after the start of illumination. As a consequence, the amount of this polypeptide increased at least 10-fold during a period of 5 h. The presence of histidine or methionine, scavengers of singlet oxygen, markedly diminished synthesis and accumulation of this polypeptide. Concomitant with the accumulation of this polypeptide on the cell surface was the appearance of an extensive array of pili.
将一种节杆菌属的细胞与亚甲蓝在光照下孵育，亚甲蓝是一种通过产生单线态氧来敏化光氧化反应的染料。细胞对这些条件的早期主要反应是刺激合成一种单一的细胞表面多肽，其质量为21,000道尔顿。这种多肽的合成速率在光照开始后约30分钟达到最高水平。因此，在5小时内这种多肽的量至少增加了10倍。单线态氧清除剂组氨酸或甲硫氨酸的存在显著减少了这种多肽的合成和积累。随着这种多肽在细胞表面的积累，出现了大量的菌毛。
