“二氨基庚二酸原生质球”中缺乏溶原性诱导。
Lack of lysogenic induction in "diaminopimelic acid spheroplasts".
作者信息
Bailone A, Levine A
出版信息
J Bacteriol. 1978 Oct;136(1):441-3. doi: 10.1128/jb.136.1.441-443.1978.
Abstract
As part of an attempt to develop a semi-in vitro system of lysogenic induction, using spheroplasts of Escherichia coli K-12 lysogenic for prophage lambda, we prepared spheroplasts by depriving E. coli dap of diaminopimelic acid (DAP-spheroplasts). DAP-spheroplasts made from E. coli (lambda cI857) were thermally inducible. However, DAP-spheroplasts of E. coli (lambda) were not inducible by UV light. Thus, it appears that a functional cell wall is required for UV induction of prophage lambda.
摘要
作为开发一种溶原性诱导半体外系统尝试的一部分，我们使用携带λ原噬菌体的大肠杆菌K-12溶原菌的原生质球，通过剥夺大肠杆菌二氨基庚二酸（DAP-原生质球）来制备原生质球。由大肠杆菌（λcI857）制成的DAP-原生质球可热诱导。然而，大肠杆菌（λ）的DAP-原生质球不能被紫外线诱导。因此，看来紫外线诱导λ原噬菌体需要有功能的细胞壁。
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