纳苏果蝇指名亚种和纳苏果蝇白变种的卫星DNA：在多线染色体和中期染色体中的定位

Satellite DNA of Drosophila nasuta nasuta and D. n. albomicana: localization in polytene and metaphase chromosomes.

作者信息

Ranganath H A, Schmidt E R, Hägele K

出版信息

Chromosoma. 1982;85(3):361-8. doi: 10.1007/BF00330358.

Abstract

The DNA from the two Drosophila nasuta races, D. n. nasuta and D. n. albomicana was investigated by CsCl density gradient centrifugation. D. n. nasuta has one major AT-rich satellite DNA sequence with a density of 1.664 g/cm3, while D. n. albomicana has at least three satellites with densities of 1.674 g/cm3, 1.665 g/cm3 and 1.661 g/cm3. The isolated satellite sequences hybridize in situ to all heterochromatic regions of all metaphase chromosomes of both races. In polytene chromosomes the satellite sequences hybridize exclusively to the chromocenter. All chromosomal regions hybridizing with the satellites show also bright quinacrine fluorescence.

摘要

通过氯化铯密度梯度离心法对两种纳氏果蝇（Drosophila nasuta）种群，即纳氏果蝇指名亚种（D. n. nasuta）和纳氏果蝇白变种（D. n. albomicana）的DNA进行了研究。纳氏果蝇指名亚种有一个主要的富含AT的卫星DNA序列，密度为1.664 g/cm³，而纳氏果蝇白变种至少有三个卫星序列，密度分别为1.674 g/cm³、1.665 g/cm³和1.661 g/cm³。分离出的卫星序列原位杂交到两个种群所有中期染色体的所有异染色质区域。在多线染色体中，卫星序列仅与染色中心杂交。所有与卫星序列杂交的染色体区域也显示出明亮的喹吖因荧光。