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果蝇鼻状卫星DNA在中期染色体中的定位。

Localization of Drosophila nasutoides satellite DNAs in metaphase chromosomes.

作者信息

Wheeler L L, Arrighi F, Cordeiro-Stone M, Lee C S

出版信息

Chromosoma. 1978 Dec 21;70(1):41-50. doi: 10.1007/BF00292214.

DOI:10.1007/BF00292214
PMID:738168
Abstract

Metaphase chromosomes of D. nasutoides were hybridized in situ with 3H-cRNA synthesized from the four satellites which make up 50--60% of the total DNA of this species. All four satellites were localized in the large, metacentric, heterochromatic chromosome four. They did not, however, appear to hybridize to centromeric or other constitutive heterochromatin, nor did they, with the exception of satellite I, seem to hybridize in the specific regions of chromosome four which, on the basis of C, Q, and H banding and AT contents, were predicted to contain some of these satellites.--Comparison of grain patterns with the results of fluorescent staining indicated that satellite-bearing heterochromatin was not always associated with other fractions of constitutive heterochromatin in interphase nuclei and was, at least partially, decondensed in some larger nuclei.

摘要

用从构成该物种总DNA 50 - 60%的四种卫星DNA合成的3H - cRNA,对纳氏果蝇的中期染色体进行原位杂交。所有四种卫星DNA都定位在大的、中着丝粒的、异染色质的第四条染色体上。然而,它们似乎并未与着丝粒或其他组成型异染色质杂交,除了卫星I外,它们似乎也未在第四条染色体的特定区域杂交,根据C、Q和H带型以及AT含量预测,这些区域应含有一些卫星DNA。——将银粒模式与荧光染色结果进行比较表明,携带卫星DNA的异染色质在间期核中并不总是与组成型异染色质的其他部分相关联,并且在一些较大的核中至少部分地发生了解聚。

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本文引用的文献

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The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus.红背无肺螈(Plethodon c. cinereus)睾丸中一种重卫星DNA的染色体定位。
Chromosoma. 1971;33(2):167-82. doi: 10.1007/BF00285631.
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Chromosomal localization of mouse satellite DNA.小鼠卫星DNA的染色体定位
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Heterochromatic nature of W chromosome in birds.鸟类W染色体的异染色质性质。
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Localisation of satellite DNA in the microchromosomes of the Japanese quail by in situ hybridization.通过原位杂交对日本鹌鹑微染色体中卫星DNA进行定位。
Chromosoma. 1972;38(3):313-8. doi: 10.1007/BF00290928.
8
DNA-dependent RNA polymerase from phage T4 infected E. coli: an enzyme missing a factor required for transcription of T4 DNA.来自噬菌体T4感染的大肠杆菌的依赖DNA的RNA聚合酶:一种缺少T4 DNA转录所需因子的酶。
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The chromosomal location of human satellite DNA 3.人类卫星DNA 3的染色体定位。
Chromosoma. 1973 Jul 18;42(4):445-51. doi: 10.1007/BF00399411.
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Localization of repeated DNA sequences in Xenopus chromosomes.非洲爪蟾染色体中重复DNA序列的定位
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