Blau L, Bittman R
J Biol Chem. 1978 Dec 10;253(23):8366-8.
The binding of filipin with cholesterol in sealed and unsealed ghosts prepared from human erythrocytes and in right-side-out and inside-out vesicles prepared from ghosts follows second order kinetics (first order in each reactant). The second order rate constant of interaction of filipin with cholesterol, determined by stopped flow measurements of the initial rate, is slower in sealed ghosts than in unsealed ghosts by a factor of 2.0, whereas identical rate constants were obtained with right-side-out and inside-out vesicles. These results suggest that the cholesterol accessible to rapid reaction with filipin is distributed symmetrically between the inner and outer halves of the lipid bilayer of erythrocyte ghost membranes.
菲律宾菌素与从人红细胞制备的密封和未密封血影以及从血影制备的外翻和内翻囊泡中的胆固醇结合遵循二级动力学（每种反应物为一级）。通过对初始速率的停流测量确定，菲律宾菌素与胆固醇相互作用的二级速率常数在密封血影中比在未密封血影中慢2.0倍，而外翻和内翻囊泡的速率常数相同。这些结果表明，可与菲律宾菌素快速反应的胆固醇对称分布在红细胞血影膜脂质双层的内半层和外半层之间。
