Horie T, Ohno T, Kinoshita K
J Chromatogr. 1982 Aug 13;231(1):111-9. doi: 10.1016/s0378-4347(00)80514-9.
A procedure for the determination of tripamide and its hydroxylated metabolites in plasma, red blood cells and urine by reversed-phase high-performance liquid chromatography is described. The concentrations in red blood cells showed a monophasic decline and the half-life was 9.5 h. The concentration in red blood cells was markedly higher than that in plasma, showing that 95-98% of the drug is present in whole blood, after a dose of tripamide (90 mg) in man. The specificity and sensitivity of this procedure appear to be satisfactory for pharmacokinetic studies.
本文描述了一种采用反相高效液相色谱法测定血浆、红细胞和尿液中曲帕胺及其羟基化代谢产物的方法。红细胞中的浓度呈单相下降，半衰期为9.5小时。在人体服用一剂曲帕胺（90毫克）后，红细胞中的浓度明显高于血浆中的浓度，表明95-98%的药物存在于全血中。该方法的特异性和灵敏度对于药代动力学研究似乎是令人满意的。
