Zipser B
J Neurosci. 1982 Oct;2(10):1453-64. doi: 10.1523/JNEUROSCI.02-10-01453.1982.
Monoclonal antibodies were used to map the distribution of neurons in the leech which contain a particular antigen. This technique reveals the genetically determined variation in cell body distribution along the nerve cord. In addition, antibodies also reveal developmental deviations, such as the occurrence of supernumerary cell bodies. Three antibodies that bind either to single types or small sets of different neurons are used to construct complete distribution patterns of antigenically related cells. Three other antibodies are used to create cell body distribution maps of antigenically homologous primary mechanosensory cells responding to noxious or pressure stimulation which form a subset of the cells stained by the antibody. Furthermore, antibodies against the pressure cells helped in the location of two different specific antigens for the same identified nerve cell.
单克隆抗体被用于绘制水蛭中含有特定抗原的神经元分布图谱。这项技术揭示了沿神经索细胞体分布的基因决定的变异。此外，抗体还揭示了发育偏差，例如额外细胞体的出现。三种分别与单一类型或少量不同神经元结合的抗体被用于构建抗原相关细胞的完整分布图。另外三种抗体被用于创建对抗有害或压力刺激的抗原同源初级机械感觉细胞的细胞体分布图，这些细胞是被该抗体染色的细胞的一个子集。此外，针对压力细胞的抗体有助于确定同一个已识别神经细胞的两种不同特异性抗原的位置。
