Oestreicher P, Cousins R J
J Nutr. 1982 Oct;112(10):1978-82. doi: 10.1093/jn/112.10.1978.
An isolated, vascularly perfused rat intestine system was utilized to compare the influence of a variety of potential zinc-binding compounds on zinc absorption. Citric acid, cysteine, reduced glutathione, histidine, methionine, picolinic acid and tryptophan were added to the lumen perfusate at 110 microM. Two lumen perfusate pH values (4.2 and 6.6) were compared. The influence of EDTA at pH 6.6 was also investigated as was the influence of citric acid, histidine, picolinic acid and tryptophan additions to the lumen perfusate at 550 microM. Of the substances tested only EDTA significantly enhanced the transfer of zinc from the lumen to the portal circulation. These results suggest these potential zinc chelators, exclusive of EDTA, do not promote zinc absorption by the intestine in this system.
利用一个孤立的、血管灌注的大鼠肠道系统来比较各种潜在的锌结合化合物对锌吸收的影响。将柠檬酸、半胱氨酸、还原型谷胱甘肽、组氨酸、蛋氨酸、吡啶甲酸和色氨酸以110微摩尔的浓度添加到肠腔灌注液中。比较了两种肠腔灌注液pH值（4.2和6.6）。还研究了pH值为6.6时EDTA的影响，以及以550微摩尔的浓度向肠腔灌注液中添加柠檬酸、组氨酸、吡啶甲酸和色氨酸的影响。在所测试的物质中，只有EDTA显著增强了锌从肠腔到门静脉循环的转运。这些结果表明，除EDTA外，这些潜在的锌螯合剂在该系统中不会促进肠道对锌的吸收。
