Bökesoy T A, Soydan S, Palaoğlu O
J Pharmacol Methods. 1982 Aug;8(1):1-7. doi: 10.1016/0160-5402(82)90002-x.
A retrograde superfusion technique is described for spontaneously beating guinea pig right atrium where both force of contraction and rate are recorded simultaneously. Drugs are applied to the system in bolus injections and the concentrations attained in the organ bath after these injections are computed through an analog system by means of measuring injected dye dilutions in a spectrophotometer. Actions of histamine and histamine H2-receptor agonists 4-methyl histamine, dimaprit, and impromidine on the preparation for the mentioned parameters are investigated. By comparing responses to bolus injections with infusions of agonists, it is concluded that all four agonists tested are rapidly passing to the biophase, and the system can be used as an alternative for the classical organ bath. Dose-response characteristics of the tested agonists are satisfactory and affinity values found are in agreement with the values reported for bathed systems.
本文描述了一种用于豚鼠右心房自搏组织的逆行超灌流技术，该技术可同时记录收缩力和心率。通过单次注射给药，利用分光光度计测量注入染料的稀释度，经模拟系统计算每次注射后器官浴中达到的药物浓度。研究了组胺及组胺H2受体激动剂4-甲基组胺、二甲双胍和英普咪定对上述参数的影响。通过比较单次注射激动剂与输注激动剂的反应，得出所有四种受试激动剂均能快速进入生物相的结论，该系统可作为传统器官浴的替代方法。受试激动剂的剂量-反应特性良好，所测得的亲和力值与报道的浸泡系统的值一致。
