Albanese R
Mutat Res. 1982 Aug;97(4):315-26. doi: 10.1016/0165-1161(82)90030-9.
Male mice were treated with methyl methanesulphonate (MMS) and then serially mated to females in oestrus, over the whole of the spermatogenic cycle. Chromosome preparations were made from fertilised eggs obtained from the matings and cultured overnight in the presence of a mititic inhibitor. No chromosomally abnormal eggs were found in matings using untreated animals but matings involving MMS-treated males produced a variety of abnormalities. The most sensitive stage in the spermatogenic cycle was 8 days after treatment, corresponding to the testicular sperm stage of spermatogenesis. At this sampling time 97% of the eggs analysed were chromosomally abnormal and the aberrations detected were predominantly 'shattered' male chromosomes. The aberration frequency in the post-meiotic stages decreased steadily up to day 20. No further structural chromosome aberrations were detected, until day 48, when chromosome fragments were detected in 2 eggs (4%) indicating that pre-meiotic damage can be induced and transmitted. The low background frequency obtained with the procedures used in this study enhances the sensitivity of the system for experimentally assessing the effects of clastogenic agents on male and female germ cells.
将雄性小鼠用甲基磺酸甲酯（MMS）处理，然后在整个生精周期内与处于发情期的雌性小鼠连续交配。从交配获得的受精卵制备染色体标本，并在有丝分裂抑制剂存在的情况下培养过夜。在使用未处理动物的交配中未发现染色体异常的卵子，但涉及经MMS处理的雄性小鼠的交配产生了多种异常情况。生精周期中最敏感的阶段是处理后8天，对应于精子发生的睾丸精子阶段。在这个取样时间，分析的卵子中有97%染色体异常，检测到的畸变主要是“破碎”的雄性染色体。减数分裂后阶段的畸变频率在第20天前稳步下降。直到第48天，才检测到进一步的结构染色体畸变，当时在2个卵子（4%）中检测到染色体片段，这表明减数分裂前的损伤可以被诱导并传递。本研究中使用的方法所获得的低背景频率提高了该系统在实验评估致裂剂对雄性和雌性生殖细胞影响方面的敏感性。
