Blank N K, Seil F J, Herndon R M
Neuroscience. 1982 Jun;7(6):1509-31. doi: 10.1016/0306-4522(82)90261-5.
Mouse-derived cerebellar explants were exposed for 5 days to cytosine arabinoside, an inhibitor of deoxyribonucleic acid synthesis. They were then maintained in normal nutrient medium until fixation for electron microscopy at 15-20 days in vitro. The cerebellar cortex lacked granule cells, but Purkinje cells, Golgi neurons and a few basket and stellate cells survived. Astrocytes and oligodendrocytes were diminished in number and myelination was absent. Purkinje cell recurrent axon collaterals increased in number and formed synapses with the surviving cortical neurons and their processes. The ultrastructural alterations that occurred in the cytosine arabinoside-treated cultures were consistent with an interpretation of cortical remodeling in which Purkinje cell axon collaterals were the dominant inhibitory elements.
将源自小鼠的小脑外植体暴露于阿糖胞苷（一种脱氧核糖核酸合成抑制剂）5天。然后将它们置于正常营养培养基中，直至在体外培养15 - 20天时进行电子显微镜固定。小脑皮质缺乏颗粒细胞，但浦肯野细胞、高尔基神经元以及一些篮状细胞和星状细胞存活了下来。星形胶质细胞和少突胶质细胞数量减少，且无髓鞘形成。浦肯野细胞的返回轴突侧支数量增加，并与存活的皮质神经元及其突起形成突触。在经阿糖胞苷处理的培养物中发生的超微结构改变与一种皮质重塑的解释一致，其中浦肯野细胞轴突侧支是主要的抑制性元件。
