[两种鼠类肿瘤病毒中蛋白激酶活性的证据]

[Evidence of protein kinase activity in 2 murine oncornaviruses].

作者信息

Blaas D, Rucheton M, Jeanteur P

出版信息

C R Acad Hebd Seances Acad Sci D. 1977 Jul 18;285(3):269-72.

Abstract

A protein kinase activity has been detected in two strains of murine Oncornaviruses, MSV/MLV and EFV. This activity phosphorylates not only endogenous viral proteins but also exogenous substrates (histones and phosvitin). The stimulation of enzyme activity by detergents along with the increase of specific activity in viruses treated with trypsin during purification suggest that the enzyme is located in the viral particle.

摘要

在两株鼠源肿瘤病毒，即莫洛尼氏肉瘤病毒/莫洛尼氏白血病病毒（MSV/MLV）和艾伯斯坦-巴尔病毒（EFV）中检测到了一种蛋白激酶活性。这种活性不仅能使内源性病毒蛋白磷酸化，还能使外源性底物（组蛋白和卵黄高磷蛋白）磷酸化。去污剂对酶活性的刺激，以及在纯化过程中用胰蛋白酶处理的病毒中比活性的增加，表明该酶位于病毒颗粒中。