Sen A, Todaro G J
Cell. 1979 Jun;17(2):347-56. doi: 10.1016/0092-8674(79)90161-2.
A low molecular weight (LMW) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. Sephadex G-75 gel filtration and affinity chromatography on actin-Sepharose conjugates allow a significant level of purification of this enzyme. The enzyme associates with microtubular proteins and inhibits the in vitro polymerization of microtubules. This study represents the first isolation of a sarcoma virus-associated protein that possesses the ability to interact directly with two major components of the cytoskeletal system.
一种与肌动蛋白结合的低分子量（LMW）蛋白磷酸激酶已从小鼠肉瘤病毒颗粒中分离出来；这种激酶活性不存在于非转化性小鼠白血病病毒中。通过Sephadex G-75凝胶过滤和肌动蛋白-琼脂糖偶联物上的亲和层析可以对这种酶进行显著程度的纯化。该酶与微管蛋白结合并抑制微管在体外的聚合。这项研究首次分离出一种具有与细胞骨架系统的两个主要成分直接相互作用能力的肉瘤病毒相关蛋白。
