Halsall H B, Kirley T L, Friedman M L
Prep Biochem. 1982;12(2):111-20. doi: 10.1080/00327488208065556.
A gel chromatographic and ion exchange procedure is described for the preparation of orosomucoid from nephrotic urine. The removal of endogenous lipid and glycosaminoglycan than yields a highly homogeneous preparation. Aggregates are formed at ambient temperatures and as a result of lyophilisation of ultrafiltration.
描述了一种从肾病尿液中制备类粘蛋白的凝胶色谱和离子交换方法。去除内源性脂质和糖胺聚糖后可得到高度均一的制剂。在环境温度下以及超滤冻干过程中会形成聚集体。