Jess G, Jülich W D, Weuffen W, Löber G
Pharmazie. 1982 Jun;37(6):420-3.
By means of fluorescence and absorption measurements the authors studied the interaction of the protein human serum albumin with relatively low concentrations (up to 5 mmol/l at the most) of thiocyanate ions. Such concentrations lead to a slight quenching of the tryptophan fluorescence in human serum albumin. The effect of thiocyanate is not due to an overall action on the protein (as sometimes observed with higher thiocyanate concentrations), but to a local interaction of the fluorophore tryptophan with the neighbouring anion-binding site which is occupied by thiocyanate.
作者通过荧光和吸收测量研究了人血清白蛋白与相对低浓度（最高达5 mmol/l）硫氰酸根离子的相互作用。这种浓度会导致人血清白蛋白中色氨酸荧光略有淬灭。硫氰酸盐的作用并非源于对蛋白质的整体作用（高硫氰酸盐浓度时有时会观察到这种情况），而是由于荧光团色氨酸与被硫氰酸盐占据的相邻阴离子结合位点的局部相互作用。
