Bässler K H, Wagner K, Schönerstedt B
J Clin Chem Clin Biochem. 1978 Oct;16(10):547-50.
An enzymatic endpoint determination of xylitol and sorbitol by the use of L-iditol dehydrogenase is described. Despite the use of pH 9 and the addition of semicarbazide, the reaction goes only 80% to completion. Therefore a standard value has to be used. The lower limit for measurement of concentrations in serum is around 0.1 mmol/l. The most favorable range is between 0.4 and 1.2 mmol/l with a coefficient of variation of below 4%. Sensitivity and precision is higher than with the kinetic method described earlier. Further simplification for the routine control of infusion therapy with polyols is suggested.
本文描述了一种使用L-艾杜糖醇脱氢酶对木糖醇和山梨醇进行酶促终点测定的方法。尽管使用了pH 9并添加了氨基脲，但反应仅进行到80%。因此必须使用标准值。血清中浓度测量的下限约为0.1 mmol/l。最适宜的范围是0.4至1.2 mmol/l，变异系数低于4%。其灵敏度和精密度高于之前描述的动力学方法。建议对多元醇输液治疗的常规控制进行进一步简化。
