Bliokh Zh L, Platonov A E, Smolianinov V V
Tsitologiia. 1982 Jun;24(6):692-8.
The adhesion of neoplastic mouse fibroblasts of line L was investigated during the formation of initial cell-substrate contact after the settling of spherical cells from suspension. nu (t), part of cell population which remained attached to the substrate after the impulse action of detaching force in the moment t, is used as a quantitative measure of the initial attachment process. The effects of the detaching force value and of the experimental medium composition on the main parameters of sigmoidal function nu (t) are studied. It is shown that 1) with the change of the detachment force three main parameters of function nu (t) are changed; 2) with the increase of the concentration of serum added in the Eagle medium the cell attachment becomes monotonely weaker and slower; albumin has the similar effect.
在球形细胞从悬浮液沉降后初始细胞 - 底物接触形成过程中，对L系肿瘤小鼠成纤维细胞的黏附进行了研究。用ν(t)（在时刻t受到分离力脉冲作用后仍附着于底物的细胞群体部分）作为初始附着过程的定量指标。研究了分离力值和实验培养基组成对S形函数ν(t)主要参数的影响。结果表明：1）随着分离力的变化，函数ν(t)的三个主要参数发生改变；2）随着添加到伊格尔培养基中血清浓度的增加，细胞黏附变得单调减弱且变慢；白蛋白有类似作用。
