Pinchuk V G, Livshits V L, Tregubova N A
Tsitologiia. 1983 May;25(5):522-6.
The ultrastructure of lipoblast-like cells produced by clones of transformed culture of strain in the medium with an increased (60%) concentration of bovine serum was studied. The aim of using the stimulator rich in adipogenous factors was the elucidation of the origin of lipid accumulation in some of cell types during heterogeneous differentiation of clones. In these conditions non-differentiated lipoblasts assumed the fine structure of mature lipid cells. The statement of the marker significance of lipid accumulation in non-differentiated cells of the clones and the conversion of these cells under the influence of non-specific stimulator to the mature lipocytes confirms the data on conservation of over-all cytogenetic potentials and the capacity of their realization in the cells of transformed cultures. It is suggested that the increased concentration of bovine serum in the cultural medium not only stimulates differentiation (by any way determined cells), but is also capable of acting on the stem elements produced by the clone. In that case the influence of the stimulator on the trends of heterogeneous differentiation is not unlikely.
研究了在牛血清浓度增加（60%）的培养基中，菌株转化培养克隆所产生的成脂母细胞样细胞的超微结构。使用富含脂肪生成因子的刺激物的目的是阐明克隆异质性分化过程中某些细胞类型脂质积累的起源。在这些条件下，未分化的成脂母细胞呈现出成熟脂质细胞的精细结构。克隆未分化细胞中脂质积累的标记意义以及这些细胞在非特异性刺激物影响下向成熟脂肪细胞的转化，证实了关于整体细胞遗传学潜能保守性及其在转化培养细胞中实现能力的数据。有人认为，培养基中牛血清浓度的增加不仅刺激分化（无论通过何种方式决定细胞），而且还能够作用于克隆产生的干细胞成分。在这种情况下，刺激物对异质性分化趋势的影响并非不可能。
