Bertolini M J
Vox Sang. 1982;43(2):87-90.
Commercially prepared fraction V products (5 and 25% albumin and 5% plasma protein fraction) and immune serum globulins were subjected to analytical molecular sieve chromatography by high performance liquid chromatography. Quantitation was performed by UV absorbance and by the Lowry protein assay. The proteins eluting in the void volume from the immune serum globulin samples yield equivalent results by UV absorbance and by Lowry assay. Proteins eluting in the void volume from fraction V products indicate approximately twice as much protein by UV absorbance when compared to Lowry protein assay.
市售的V组分产品（5%和25%白蛋白以及5%血浆蛋白组分）和免疫血清球蛋白通过高效液相色谱法进行分析性分子筛色谱分析。通过紫外吸光度和洛瑞蛋白测定法进行定量。从免疫血清球蛋白样品的空体积中洗脱出来的蛋白质，通过紫外吸光度和洛瑞测定法得到的结果相当。与洛瑞蛋白测定法相比，从V组分产品的空体积中洗脱出来的蛋白质，通过紫外吸光度测定显示的蛋白质量大约是其两倍。
