来自患有恶性淋巴瘤的狒狒造血器官细胞的连续淋巴母细胞悬浮培养物。报告III：免疫学研究。

Continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma. Report III: Immunological studies.

作者信息

Kokosha L V, Agrba V Z, Lapin B A, Yakovleva L A, Arshba N N, Markova T P, Pimanovskaja V V

出版信息

Exp Pathol (Jena). 1977;13(4-5):247-54. doi: 10.1016/s0014-4908(77)80009-4.

Abstract

Lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the B-cell type. Cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human Burkitt's lymphoma patients with antibody titer to capsid antigen of Epstein-Barr virus 1: 320. There is a correlation between the number of antigen-containing cells in these cultures and the number of cells in which herpes virus is revealed electron microscopically. Sera of baboons with lymphoma contain high titers of antibodies to Epstein-Barr (EBV) virus. Specific differences between antigens being produced in cultures by HBV (herpes virus of baboon) and EBV (Epstein-Barr virus) are minimal, that indicates their immunologic similarity.

摘要

从患淋巴瘤的狒狒造血器官建立的淋巴母细胞样培养物属于B细胞类型。使用来自人伯基特淋巴瘤患者且对爱泼斯坦 - 巴尔病毒衣壳抗原抗体效价为1:320的血清，通过间接免疫荧光反应，在从患淋巴瘤的狒狒建立的悬浮培养细胞中，2% - 6%的细胞可显示出细胞质抗原。这些培养物中含抗原细胞的数量与通过电子显微镜发现疱疹病毒的细胞数量之间存在相关性。患淋巴瘤的狒狒血清中含有高滴度的抗爱泼斯坦 - 巴尔（EBV）病毒抗体。由狒狒疱疹病毒（HBV）和爱泼斯坦 - 巴尔病毒（EBV）在培养物中产生的抗原之间的特异性差异极小，这表明它们在免疫学上具有相似性。