Zikán J, Dráber P
Folia Biol (Praha). 1977;23(4):243-8.
Sarcoma I cells from ascitic fluid of A/Ph mice were modified with iodoacetamide, diketene and glutaraldehyde. Syngeneic and allogeneic mice were immunized with the modified cells and the effect of this immunization on the growth or rejection of the native sarcoma injected intramuscularly into the hind leg was ascertained. The antitumour effect of the modified cells was compared with that of the irradiated ones. Only cells treated with iodoacetamide gave a better antitumour effect than the unmodified cells irradiated with a minimal dose of gamma irradiation capable of stopping the proliferation of these cells.
用碘乙酰胺、双乙烯酮和戊二醛对A/Ph小鼠腹水的肉瘤I细胞进行修饰。用修饰后的细胞对同基因和异基因小鼠进行免疫，并确定这种免疫对注射到后腿肌肉中的天然肉瘤生长或排斥的影响。将修饰细胞的抗肿瘤效果与辐照细胞的效果进行比较。只有用碘乙酰胺处理的细胞比用能阻止这些细胞增殖的最小剂量γ射线辐照的未修饰细胞具有更好的抗肿瘤效果。
