Zolese G, Curatola G
Boll Soc Ital Biol Sper. 1982 Jul 30;58(14):882-6.
The fluorescence of indole ring of tryptophan is sensitive to the characteristics of the solvent in which it is located. So that is can be used as a probe of its microenvironment. In the present report we have studied fluorescence changes of tryptophan molecule located in multilamellar liposome of dipalmitoyl-lecithin (DPL). The temperature dependence of tryptophan fluorescence intensity shows increase related to the phase changes in DPL-liposomes. n-Butanol, a fluidizing agent, induces a linear dependence of tryptophan fluorescence from temperature.
色氨酸吲哚环的荧光对其所处溶剂的特性敏感。因此它可用作其微环境的探针。在本报告中,我们研究了位于二棕榈酰卵磷脂(DPL)多层脂质体中的色氨酸分子的荧光变化。色氨酸荧光强度的温度依赖性显示出与DPL脂质体中的相变相关的增加。正丁醇,一种流化剂,导致色氨酸荧光与温度呈线性关系。