Nikushkin E V, Klebanov G I, Vladimirov Iu A
Biofizika. 1977 Nov-Dec;22(6):1049-55.
Phase transition induced by temperature changes in liposomes prepared from synthetic dimyristoyl-(DML), dipalmytoyl-(DPL) and distearyl-(DSL) lecithins and also from equimolar mixture of DML and DPL and mixtures of DPL and cholesterol were studied with fluorescent probes such as 3-metoxybenzantron (MBA) and dimetylaminocholkone (DMC). The significant changes of fluorescence intensity and maximum position of MBA and DMC were found in the regions of phase transitions at 23, 42, 54 and 31c in liposomes from DML, DPL, DSL and mixture of DML and DPL, respectively. Cholesterol incorporation into liposomes from DPL led to a decrease of transition temperature and cooperativity for these liposomes. At cholesterol concentration of 20 mol. percent or more the transition disappeared completely. It is concluded that MBA and DMC can be used for the investigation of biological membranes phase transitions.
利用3 - 甲氧基苯并蒽（MBA）和二甲基氨基苯甲酮（DMC）等荧光探针，研究了由合成的二肉豆蔻酰磷脂（DML）、二棕榈酰磷脂（DPL）和二硬脂酰磷脂（DSL）制备的脂质体以及DML与DPL的等摩尔混合物和DPL与胆固醇的混合物，在温度变化时的相变情况。分别在由DML、DPL、DSL以及DML与DPL混合物制备的脂质体中，于23℃、42℃、54℃和31℃的相变区域，发现了MBA和DMC荧光强度及最大位置的显著变化。向由DPL制备的脂质体中掺入胆固醇，会导致这些脂质体的转变温度和协同性降低。当胆固醇浓度达到20摩尔百分比或更高时，相变完全消失。得出的结论是，MBA和DMC可用于研究生物膜的相变。
