Caranobe C, Sie P, Boneu B
Br J Haematol. 1982 Oct;52(2):253-8. doi: 10.1111/j.1365-2141.1982.tb03887.x.
Serotonin uptake and storage were studied in human platelet density subpopulations which were isolated after isopycnic centrifugation on a discontinuous iso-osmolar stractan gradient. Kinetic parameters of the serotonin uptake were calculated (Km, Vi max) and the granular storage capacity was determined by comparing the total amount taken up in the presence or absence of reserpine, a specific inhibitor of the uptake at the granular level. Mean platelet volume and the number of mepacrine-labelled dense bodies were also determined. The results show that the active metabolic process of serotonin uptake is identical whatever the platelet subpopulation but the storage capacity is greater in the densest fraction which contains more dense bodies than the lightest one. Thus active serotonin uptake appears as another well-defined platelet metabolic process which is independent of platelet density; this argues against the conception that light platelets could be old platelets with reduced functional capacities.
在通过在不连续等渗分层梯度上进行等密度离心分离得到的人血小板密度亚群中研究了血清素的摄取和储存。计算了血清素摄取的动力学参数（Km，最大摄取速度），并通过比较在存在或不存在利血平（颗粒水平摄取的特异性抑制剂）的情况下摄取的总量来确定颗粒储存能力。还测定了平均血小板体积和吖啶橙标记的致密体数量。结果表明，无论血小板亚群如何，血清素摄取的活跃代谢过程都是相同的，但在最致密的部分中储存能力更大，该部分比最轻的部分含有更多的致密体。因此，活跃的血清素摄取似乎是另一个明确的血小板代谢过程，它与血小板密度无关；这与轻血小板可能是功能能力降低的衰老血小板的概念相矛盾。
