致病性福氏耐格里阿米巴富含磷脂酶的培养基对人髓磷脂磷脂的降解作用

Degradation of human myelin phospholipids by phospholipase-enriched culture media of pathogenic Naegleria fowleri.

作者信息

Hysmith R M, Franson R C

出版信息

Biochim Biophys Acta. 1982 Sep 14;712(3):698-701. doi: 10.1016/0005-2760(82)90300-9.

Abstract

Cell-free media from cultures of virulent Naegleria fowleri were tested for phospholipase activities and their ability to degrade phospholipids of human myelin. Virulent N. fowleri selectively released lipolytic enzymes into the media at various times during growth and hydrolyzed the phospholipids of human myelin, while media from virulent-attenuated and nonpathogenic Naegleria spp. were almost totally inactive. Hydrolysis of myelin phospholipid increased concomitantly with amebal growth, and the relative rate of breakdown at pH 7.5 was sphingomyelin greater than phosphatidylcholine greater than phosphatidylethanolamine. Elevated levels of lysophosphatidylcholine and lysophosphatidylethanolamine were also noted.

摘要

对来自剧毒福氏耐格里阿米巴培养物的无细胞培养基进行了磷脂酶活性及其降解人髓磷脂磷脂能力的测试。剧毒福氏耐格里阿米巴在生长过程中的不同时间选择性地将脂解酶释放到培养基中，并水解人髓磷脂的磷脂，而来自毒力减弱和非致病性耐格里属阿米巴的培养基几乎完全没有活性。髓磷脂磷脂的水解随着阿米巴生长而增加，在pH 7.5时的相对分解速率为鞘磷脂大于磷脂酰胆碱大于磷脂酰乙醇胺。还注意到溶血磷脂酰胆碱和溶血磷脂酰乙醇胺水平升高。