Popov V Ia, Kurek A K, Chasovnikova L V, Matveeva N A, Lavrent'ev V V
Biofizika. 1982 Jul-Aug;27(4):604-8.
The deposited immunoglobulin G (IgG) monomolecular layers consisting of native protein molecules oriented either horizontally or vertically towards the Si internal reflection plate, and also of molecules, denatured in monolayers at air-water interface have been studied by attenuated total-reflection (ATR) IR-spectroscopy technique. It was shown that deposition of IgG molecules on Si surface from monolayers at air--water interface did not change conformation and orientation of the molecules. The value of monolayer thickness correlates with the IgG molecule dimensions calculated from X-ray diffraction data. It was also established that beta-conformation prevails in the secondary structure of both native and denaturated molecules.
通过衰减全反射（ATR）红外光谱技术研究了沉积在硅内反射板上的免疫球蛋白G（IgG）单分子层，该单分子层由水平或垂直于硅内反射板取向的天然蛋白质分子组成，还包括在空气-水界面的单分子层中变性的分子。结果表明，IgG分子从空气-水界面的单分子层沉积到硅表面时，其分子构象和取向并未改变。单分子层厚度值与根据X射线衍射数据计算出的IgG分子尺寸相关。还确定了β-构象在天然和变性分子的二级结构中均占主导。
