Chasovnikova L V, Lavrent'ev V V, Aleshkin V A, Matveeva N A, Cheredeev A N
Biull Eksp Biol Med. 1984 Jul;98(7):63-5.
The surface denaturation kinetics of mouse normal IgG and IgGl kappa secreted by myeloma MOPC-21 was studied in monomolecular layers at the air-water interface. Based on the denaturation kinetics data the orientation of the native IgG molecules was determined relative to the interface surface, which turned out to be horizontal for normal IgG and vertical for myelomic ones. As regards the orientation in the monolayers and the rate of surface denaturation, the mouse normal IgG were found to be similar to normal IgG from other species. Like human myelomic IgG, MOPC-21 IgGl kappa differed from normal IgG in both the orientation and lesser native structure stability.
在空气-水界面的单分子层中研究了小鼠正常IgG和骨髓瘤MOPC-21分泌的IgG1 κ的表面变性动力学。基于变性动力学数据，确定了天然IgG分子相对于界面表面的取向，结果表明正常IgG为水平取向，骨髓瘤IgG为垂直取向。关于单分子层中的取向和表面变性速率，发现小鼠正常IgG与其他物种的正常IgG相似。与人类骨髓瘤IgG一样，MOPC-21 IgG1 κ在取向和较低的天然结构稳定性方面均与正常IgG不同。
