Bagnoli P, Burkhalter A, Vischer A, Henke H, Cuénod M
Brain Res. 1982 Sep 16;247(2):289-302. doi: 10.1016/0006-8993(82)91253-7.
Choline acetyltransferase (ChAT) and glutamic acid decarboxylase (GAD) activities were measured in various visual structures of the pigeon brain after long-term monocular deprivation followed by short-term binocular presence or absence of light stimulation. The short-term phase (45 min) was coupled with a 2-deoxyglucose experiment in order to select the adequate brain samples. After mononuclear deprivation during the first 6-11 months, ChAT activity was higher by 40-60% in the dorsolateral visual Wulst contralateral to the deprived eye, as compared to the other side. In the same structure, animals, either monocularly deprived or undeprived and exposed binocularly to environmental light for 45 min, had higher ChAT activities on both sides than those maintained in the dark. Mononuclear deprivation performed in adult animals did not affect the ChAT activity in visual Wulst. GAD activity was bilaterally decreased in the visual Wulst after early monocular deprivation. These results suggest that early monocular deprivation has an effect on biochemical systems involved in synaptic transmission at selected relays of the visual pathways.
在长期单眼剥夺后，接着进行短期双眼有无光刺激的情况下，测量了鸽脑不同视觉结构中的胆碱乙酰转移酶（ChAT）和谷氨酸脱羧酶（GAD）活性。短期阶段（45分钟）与2-脱氧葡萄糖实验相结合，以选择合适的脑样本。在最初6至11个月的单眼剥夺后，与另一侧相比，剥夺眼对侧的背外侧视觉中脑（Wulst）的ChAT活性高40%至60%。在相同结构中，单眼剥夺或未剥夺且双眼暴露于环境光45分钟的动物，其两侧的ChAT活性均高于置于黑暗中的动物。成年动物进行单眼剥夺不影响视觉中脑的ChAT活性。早期单眼剥夺后，视觉中脑的GAD活性双侧降低。这些结果表明，早期单眼剥夺对视觉通路特定中继处参与突触传递的生化系统有影响。
