Yoshida Y, Boku E, Ohkubo T, Kamamoto T, Hiraoka A, Yamagishi M, Uchino H
Cell Tissue Kinet. 1982 Sep;15(5):565-73. doi: 10.1111/j.1365-2184.1982.tb01578.x.
DNA labelling by [3H]thymidine and the sandwich radioimmunolabelling method were used to characterize marrow lymphoid cells and to study the kinetics of production and maturation of small lymphocytes in the bone marrow of adult mice. Marrow lymphoid cells consisted of non-proliferating small lymphocytes, 30-40% of which had detectable surface immunoglobulin (SmIg), and proliferating large lymphoid cells lacking SmIg. Double-labelling experiments employing [3H]thymidine in vivo followed by sandwich radioimmunolabelling in vitro indicated that marrow small lymphocytes lack detectable SmIg when they are formed but develop SmIg within the first few days after production. Marrow lymphocytopoiesis includes; (1) proliferation of large lymphoid cells, which are presumptive small lymphocyte progenitors, which have a cell cycle time of 14-15 hr, and (2) a 3-5 day intramyeloid stage when many newly formed small lymphocytes undergo maturational changes towards the B cell lineage.
采用[³H]胸腺嘧啶核苷进行DNA标记以及夹心放射免疫标记法，以鉴定骨髓淋巴样细胞，并研究成年小鼠骨髓中小淋巴细胞的产生和成熟动力学。骨髓淋巴样细胞由不增殖的小淋巴细胞组成，其中30%-40%具有可检测到的表面免疫球蛋白（SmIg），以及缺乏SmIg的增殖性大淋巴样细胞。体内采用[³H]胸腺嘧啶核苷，随后体外进行夹心放射免疫标记的双重标记实验表明，骨髓小淋巴细胞形成时缺乏可检测到的SmIg，但在产生后的头几天内会发育出SmIg。骨髓淋巴细胞生成包括：（1）大淋巴样细胞的增殖，大淋巴样细胞是假定的小淋巴细胞祖细胞，其细胞周期时间为14-15小时，以及（2）一个3-5天的髓内阶段，在此期间许多新形成的小淋巴细胞朝着B细胞谱系发生成熟变化。
