Nakamura J, Yakata M
Clin Chem. 1982 Nov;28(11):2294-6.
Our examination of urine components separated by gel filtration revealed the presence of an inhibitor that decreases the analytical recovery of protein in a turbidimetric assay involving sulfosalicylic acid as reagent (Proc. Soc. Exp. Biol. Med. 92: 748, 1956). The apparent relative molecular mass of this inhibitor was in the range 160 000-240 000. A study with purified proteins showed a similar inhibition by gamma-globulin, glycoprotein, and beta-lipoprotein in the assay of albumin by the same turbidimetric method. In contrast, measurement of protein by a dye binding method was not affected by these materials. The low values for apparent urinary protein given by the turbidimetric method as compared with those by the dye-binding method are at least partly ascribable to the inhibitor.
我们对通过凝胶过滤分离出的尿液成分进行的检查显示，存在一种抑制剂，该抑制剂会降低在以磺基水杨酸作为试剂的比浊法测定蛋白质时的分析回收率（《实验生物学与医学学会会刊》92: 748, 1956）。这种抑制剂的表观相对分子质量在160000 - 240000范围内。对纯化蛋白质的研究表明，在通过相同比浊法测定白蛋白时，γ-球蛋白、糖蛋白和β-脂蛋白也有类似的抑制作用。相比之下，通过染料结合法测定蛋白质不受这些物质的影响。与染料结合法相比，比浊法给出的表观尿蛋白低值至少部分归因于该抑制剂。
