Papadopoulos G K, Muccio D D, Hsiao T L, Cassim J Y
J Membr Biol. 1978 Oct 19;43(2-3):277-94. doi: 10.1007/BF01933483.
Direct comparison of the absorption and circular dichroic spectra of dark- and light-adapted purple membrane from Halobacterium cutirubrum and Halobacterium halobium indicated no apparent species differences. In addition, sequential bleaching and regeneration of the purple membrane with concomitant monitoring of the absorption and circular dichroic spectra showed no species differences as well. Furthermore, perturbation of the structure of the purple membrane from either species with a detergent, Triton X-100, yielded similar spectral changes. It was concluded: (i) no apparent differences exist in the molecular organization and protein fine structure of the two purple membranes, (ii) if exciton interaction among the retinal chromophores is a reasonable possibility in the case of the purple membrane from Halobacterium halobium, it must be similarly so for the membrane from Halobacterium cutirubrum, (iii) the effects of light adaptation on the membrane structure of both species are essentially the same, and (iv) the underlying molecular mechanisms for the bleaching and regenerative processes must be similar, if not identical, for the purple membranes of the two species.
对来自红皮盐杆菌和盐生盐杆菌的暗适应和光适应紫膜的吸收光谱和圆二色光谱进行直接比较，结果表明没有明显的物种差异。此外，对紫膜进行连续漂白和再生，并同时监测吸收光谱和圆二色光谱，也未发现物种差异。此外，用去污剂Triton X-100对这两种物种的紫膜结构进行扰动，产生了相似的光谱变化。得出以下结论：(i) 两种紫膜的分子组织和蛋白质精细结构没有明显差异；(ii) 如果在盐生盐杆菌紫膜的情况下，视黄醛发色团之间存在激子相互作用是合理的可能性，那么对于红皮盐杆菌的膜也必然如此；(iii) 光适应对这两种物种膜结构的影响基本相同；(iv) 对于这两种物种的紫膜，漂白和再生过程的潜在分子机制即使不完全相同，也必然相似。
