• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Molecular weight of bacteriorhodopsin solubilized in Triton X-100.溶解于曲拉通X-100中的细菌视紫红质的分子量。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2803-4. doi: 10.1073/pnas.74.7.2803.
2
Comparative studies on the fine structure of purple membrane from Halobacterium Cutirubrum and Halobacterium Halobium.嗜盐红菌和嗜盐栖热菌紫膜精细结构的比较研究。
J Membr Biol. 1978 Oct 19;43(2-3):277-94. doi: 10.1007/BF01933483.
3
Formation and properties of bacteriorhodopsin monomers in the non-ionic detergents octyl-beta-D-glucoside and Triton X-100.在非离子去污剂辛基-β-D-葡萄糖苷和吐温X-100中细菌视紫红质单体的形成与性质
FEBS Lett. 1978 Dec 15;96(2):322-6. doi: 10.1016/0014-5793(78)80427-x.
4
Light-dark adaptation of bacteriorhodopsin in triton-treated purple membrane.经曲通处理的紫膜中细菌视紫红质的明暗适应
Biochim Biophys Acta. 1980 Mar 7;590(1):13-23. doi: 10.1016/0005-2728(80)90142-5.
5
Isoelectric forms of bacteriorhodopsin from Halobacterium halobium.
Biochim Biophys Acta. 1981 Aug 28;670(1):32-8. doi: 10.1016/0005-2795(81)90045-3.
6
Effect of Triton X-100 and of deuteration on the amplitude of the O640-intermediate in the bacteriorhodopsin photocycle.
FEBS Lett. 1981 Jun 15;128(2):249-54. doi: 10.1016/0014-5793(81)80092-0.
7
Solubilization steps of dark-adapted purple membrane by Triton X-100. A spectroscopic study.
FEBS Lett. 1992 Jul 6;305(3):249-53. doi: 10.1016/0014-5793(92)80679-b.
8
Nonionic detergent effects on spectroscopic characteristics and the photocycle of bacteriorhodopsin in purple membranes.非离子去污剂对紫膜中细菌视紫红质光谱特性及光循环的影响。
Arch Biochem Biophys. 1983 Mar;221(2):557-64. doi: 10.1016/0003-9861(83)90175-3.
9
Photobleaching of bacteriorhodopsin solubilized with triton X-100.用曲拉通X-100增溶的细菌视紫红质的光漂白。
Photochem Photobiol. 2005 Sep-Oct;81(5):1131-7. doi: 10.1562/2005-03-22-RA-470.
10
Refolding and proton pumping activity of a polyethylene glycol-bacteriorhodopsin water-soluble conjugate.聚乙二醇-细菌视紫红质水溶性缀合物的复性及质子泵浦活性
Protein Sci. 1993 Jul;2(7):1161-70. doi: 10.1002/pro.5560020711.

引用本文的文献

1
Spiking Neurons Derived from Proteinoid and Bacteriorhodopsin.源自类蛋白质和细菌视紫红质的脉冲神经元。
ACS Appl Bio Mater. 2025 Sep 15;8(9):7953-7978. doi: 10.1021/acsabm.5c00964. Epub 2025 Aug 17.
2
Mitoxantrone-Surfactant Interactions: A Physicochemical Overview.米托蒽醌与表面活性剂的相互作用：物理化学概述
Molecules. 2016 Oct 13;21(10):1356. doi: 10.3390/molecules21101356.
3
Crystal structure of Escherichia coli-expressed Haloarcula marismortui bacteriorhodopsin I in the trimeric form.大肠杆菌表达的三聚体形式的嗜盐菌紫膜质I的晶体结构。
PLoS One. 2014 Dec 5;9(12):e112873. doi: 10.1371/journal.pone.0112873. eCollection 2014.
4
Unique biphasic band shape of the visible circular dichroism of bacteriorhodopsin in purple membrane: Excitons, multiple transitions or protein heterogeneity?菌紫质紫膜中可见圆二色性的独特双相带形状：激子、多种跃迁还是蛋白质异质性？
Biophys J. 1992 Nov;63(5):1432-42. doi: 10.1016/S0006-3495(92)81701-0.
5
Effect of membrane potential on the conformation of bacteriorhodopsin reconstituted in lipid vesicles.膜电位对脂质体中重组菌紫质构象的影响。
Biophys J. 1988 Oct;54(4):747-50. doi: 10.1016/S0006-3495(88)83011-X.
6
The directed cooperative assembly of proteorhodopsin into 2D and 3D polarized arrays.视紫质定向协同组装成二维和三维偏振阵列。
Proc Natl Acad Sci U S A. 2007 May 15;104(20):8212-7. doi: 10.1073/pnas.0702336104. Epub 2007 May 8.
7
Auxin receptors of maize coleoptile membranes do not have ATPase activity.玉米中胚轴质膜上的生长素受体没有 ATP 酶活性。
Plant Physiol. 1978 Apr;61(4):581-4. doi: 10.1104/pp.61.4.581.
8
Determination of the aggregate size in detergent solution of the light-harvesting chlorophyll a/b-protein complex from chloroplast membranes.测定叶绿体膜中捕光叶绿素 a/b 蛋白复合物在洗涤剂溶液中的聚集大小。
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3797-801. doi: 10.1073/pnas.85.11.3797.
9
Comparison of the dynamics of the primary events of bacteriorhodopsin in its trimeric and monomeric states.细菌视紫红质三聚体和单体状态下主要事件动力学的比较。
Biophys J. 2002 Sep;83(3):1557-66. doi: 10.1016/S0006-3495(02)73925-8.
10
An improved tripod amphiphile for membrane protein solubilization.一种用于膜蛋白增溶的改良三脚架两亲分子。
Protein Sci. 2000 Dec;9(12):2518-27. doi: 10.1110/ps.9.12.2518.

本文引用的文献

1
Further characterization of particulate fractions from lysed cell envelopes of Halobacterium halobium and isolation of gas vacuole membranes.嗜盐菌溶胞细胞膜颗粒组分的进一步表征及气胞膜的分离
J Cell Biol. 1968 Aug;38(2):337-57. doi: 10.1083/jcb.38.2.337.
2
Computed circular dichroism spectra for the evaluation of protein conformation.用于评估蛋白质构象的计算圆二色光谱。
Biochemistry. 1969 Oct;8(10):4108-16. doi: 10.1021/bi00838a031.
3
Structure of the purple membrane.紫膜的结构。
Nat New Biol. 1971 Sep 29;233(39):152-5. doi: 10.1038/newbio233152a0.
4
Rhodopsin-like protein from the purple membrane of Halobacterium halobium.来自嗜盐栖热菌紫膜的视紫红质样蛋白。
Nat New Biol. 1971 Sep 29;233(39):149-52. doi: 10.1038/newbio233149a0.
5
Molecular weight, shape and structure of mixed micelles of Triton X-100 and sphingomyelin.曲拉通X-100与鞘磷脂混合胶束的分子量、形状和结构
Biochim Biophys Acta. 1974 Aug 21;363(1):98-111. doi: 10.1016/0005-2736(74)90009-1.
6
Bovine rhodopsin: characterization of the complex formed with Triton X-100.牛视紫红质：与曲拉通X-100形成的复合物的特性
Eur J Biochem. 1974 May 15;44(2):383-90. doi: 10.1111/j.1432-1033.1974.tb03495.x.
7
Functions of a new photoreceptor membrane.一种新型光感受器膜的功能。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2853-7. doi: 10.1073/pnas.70.10.2853.
8
Evidence for chromophore-chromophore (exciton) interaction in the purple membrane of Halobacterium halobium.嗜盐栖热菌紫色膜中发色团-发色团（激子）相互作用的证据。
Biochem Biophys Res Commun. 1976 Mar 8;69(1):1-6. doi: 10.1016/s0006-291x(76)80263-x.
9
Photoreceptor protein from the purple membrane of Halobacterium halobium. Molecular weight and retinal binding site.来自嗜盐菌紫膜的光受体蛋白。分子量与视黄醛结合位点。
Biochemistry. 1976 Feb 24;15(4):792-8. doi: 10.1021/bi00649a010.
10
Molecular structure determination by electron microscopy of unstained crystalline specimens.通过电子显微镜对未染色晶体标本进行分子结构测定。
J Mol Biol. 1975 May 25;94(3):425-40. doi: 10.1016/0022-2836(75)90212-0.

溶解于曲拉通X-100中的细菌视紫红质的分子量。

Molecular weight of bacteriorhodopsin solubilized in Triton X-100.

作者信息

Reyenolds J A, Stoeckenius W

出版信息

Proc Natl Acad Sci U S A. 1977 Jul;74(7):2803-4. doi: 10.1073/pnas.74.7.2803.

DOI:10.1073/pnas.74.7.2803
PMID:268630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431297/
Abstract

Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin is monomeric under the conditions of solubilization with a molecular weight of 24,250+/-2,000 and binds approximately one micelle of Triton X-100.

摘要

来自嗜盐栖热菌的细菌视紫红质已溶解在非离子去污剂Triton X-100中。圆二色光谱和流体动力学性质表明，该蛋白质在去污剂中的结构与天然膜结合形式相比没有显著改变。在溶解条件下，细菌视紫红质为单体，分子量为24,250±2,000，并且结合大约一个Triton X-100胶束。