溶解于曲拉通X-100中的细菌视紫红质的分子量。
Molecular weight of bacteriorhodopsin solubilized in Triton X-100.
作者信息
Reyenolds J A, Stoeckenius W
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2803-4. doi: 10.1073/pnas.74.7.2803.
Abstract
Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin is monomeric under the conditions of solubilization with a molecular weight of 24,250+/-2,000 and binds approximately one micelle of Triton X-100.
摘要
来自嗜盐栖热菌的细菌视紫红质已溶解在非离子去污剂Triton X-100中。圆二色光谱和流体动力学性质表明，该蛋白质在去污剂中的结构与天然膜结合形式相比没有显著改变。在溶解条件下，细菌视紫红质为单体，分子量为24,250±2,000，并且结合大约一个Triton X-100胶束。
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