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一种改良的中性蔗糖离心法,用于快速检测人淋巴细胞中受γ射线损伤及修复的DNA。

A modified neutral sucrose centrifugation method for rapid detection of DNA damaged by gamma-radiation and repair in human lymphocytes.

作者信息

Pitout M J, Louw W K, Izatt H, van Vuuren C J, Hugo N, van der Watt J J

出版信息

Int J Biochem. 1982;14(10):899-904. doi: 10.1016/0020-711x(82)90073-8.

DOI:10.1016/0020-711x(82)90073-8
PMID:7128907
Abstract
  1. The influence of 60Co gamma-radiation on the sedimentation behaviour of human lymphocyte nucleoids in neutral sucrose gradients was studied with the aid of a modified fluorescent monitor system consisting of the DNA-binding dye, Hoechst 33258 and a vertical centrifuge rotor. 2. A combined method employing the sedimentation procedure and the incorporation of tritiated nucleosides was utilized to evaluate the repair of gamma-radiated DNA.
摘要
  1. 借助一种改良的荧光监测系统,该系统由DNA结合染料Hoechst 33258和一个垂直离心转子组成,研究了60Coγ辐射对人淋巴细胞核仁在中性蔗糖梯度中沉降行为的影响。2. 采用沉降程序和掺入氚化核苷的联合方法来评估γ辐射DNA的修复情况。

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A modified neutral sucrose centrifugation method for rapid detection of DNA damaged by gamma-radiation and repair in human lymphocytes.一种改良的中性蔗糖离心法,用于快速检测人淋巴细胞中受γ射线损伤及修复的DNA。
Int J Biochem. 1982;14(10):899-904. doi: 10.1016/0020-711x(82)90073-8.
2
The effect of 60Co-gamma radiation on the sedimentation behaviour of human lymphocyte nucleoids in neutral sucrose gradients.60钴-γ辐射对人淋巴细胞核仁在中性蔗糖梯度中的沉降行为的影响。
Int J Biochem. 1980;12(3):471-2. doi: 10.1016/0020-711x(80)90130-5.
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Changes in DNA supercoiling during repair of gamma-radiation-induced damage.γ辐射诱导损伤修复过程中DNA超螺旋的变化
Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Nov;52(5):693-703. doi: 10.1080/09553008714552211.
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DNA-repair synthesis in ataxia telangiectasis lymphoblastoid cells.共济失调毛细血管扩张症淋巴母细胞样细胞中的DNA修复合成
Mutat Res. 1981 Dec;84(2):419-27. doi: 10.1016/0027-5107(81)90209-8.
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