Scholnick S B, Bruns P J
Genetics. 1982 Sep;102(1):29-38. doi: 10.1093/genetics/102.1.29.
Conjugating Tetrahymena can abort the sexual cycle before the production of progeny somatic (macro-) nuclei and return to vegetative growth. We have analyzed the germinal (micronuclear) genotypes of these cells in order to determine the stage at which they aborted conjugation. Our data demonstrate that nearly all of these cells attempt meiosis, but that very few reach the successful completion of cross-fertilization. The resulting micronuclear genotypes suggest that either germinal chromosomes or entire nuclei are lost during an unsuccessful attempt at meiosis or cross-fertilization. We conclude that the decision to develop progeny macronuclei is made during meiosis and is dependent on the completion of some step necessary for successful cross-fertilization.
接合中的四膜虫可以在子代体细胞(大)核产生之前中止有性周期,并恢复营养生长。我们分析了这些细胞的生殖(微核)基因型,以确定它们中止接合的阶段。我们的数据表明,几乎所有这些细胞都尝试进行减数分裂,但很少有细胞能成功完成交叉受精。由此产生的微核基因型表明,在减数分裂或交叉受精的不成功尝试过程中,生殖染色体或整个细胞核会丢失。我们得出结论,产生子代大核的决定是在减数分裂过程中做出的,并且取决于成功交叉受精所需的某些步骤的完成。