嗜热四膜虫中对6-甲基嘌呤和环己酰亚胺的诱导抗性。I.嗜热栖热菌的种系突变体
Induced resistance to 6-methylpurine and cycloheximide in tetrahymena. I. Germ line mutants of T. thermophila.
作者信息
Byrne B C, Brussard T B, Bruns P J
出版信息
Genetics. 1978 Aug;89(4):695-702. doi: 10.1093/genetics/89.4.695.
Abstract
Two new mutant lines of Tetrahymena thermophila (T. pyriformis, syngen 1), each conferring resistance to a different agent, are described. Resistance to cycloheximide and 6-methylpurine are each determined by dominant genes, ChxA2 and Mpr; the traits show phenotypic assortment. The method used to select these mutations, the critical importance of backcrossing to wild type following mutagenesis, and the utility of these marker genes in further mutagenic selection schemes and studies of the sexual cycle of Tetrahymena are noted.
摘要
本文描述了嗜热四膜虫（梨形四膜虫，同宗配合1）的两个新突变株系，每个株系对不同药剂具有抗性。对环己酰亚胺和6-甲基嘌呤的抗性分别由显性基因ChxA2和Mpr决定；这些性状表现出表型分离。文中指出了用于选择这些突变的方法、诱变后与野生型回交的关键重要性，以及这些标记基因在进一步诱变选择方案和四膜虫有性周期研究中的实用性。
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