Schmidt K G, Rasmussen J W, Lorentzen M
Haemostasis. 1982;11(4):193-203. doi: 10.1159/000214663.
111In-labelled human platelets were aggregated with ADP and subjected to ultrastructural morphometric analysis. In addition, the hemostatic function in vivo and the ultrastructural morphology ex vivo of rabbit platelets were examined. The platelet isolation and labelling procedures exerted no certain influence on the aggregation response, and platelet surface/volume calculations did not indicate that platelet activation had taken place. Bleeding time experiments in rabbits indicated that the hemostatic effectiveness of the labelled platelets was unimpaired. Transfused 111In-labelled platelets isolated from the recipient rabbits exhibited fewer electromicroscopic signs of platelet activation than the same platelets prior to transfusion. Our results indicate that the described procedure for isolation and 111In-labelling of platelets induces only insignificant damage to the platelets.
用111铟标记的人血小板与二磷酸腺苷（ADP）聚集，并进行超微结构形态计量分析。此外，还检测了兔血小板在体内的止血功能和体外的超微结构形态。血小板分离和标记程序对聚集反应没有产生特定影响，血小板表面/体积计算结果未表明血小板已发生激活。兔的出血时间实验表明，标记血小板的止血效果未受损害。从受体兔分离出的经111铟标记并回输的血小板，与输血前相比，其血小板激活的电镜征象较少。我们的结果表明，所描述的血小板分离和111铟标记程序仅对血小板造成轻微损伤。
