Isaka Y, Kimura K, Matsumoto M, Kamada T, Imaizumi M
Department of Cardiovascular Medicine and Radiological Science, Osaka National Hospital, Japan.
Eur J Nucl Med. 1991;18(5):326-31. doi: 10.1007/BF02285460.
Human platelets were labelled in the absence or presence of plasma using indium-111 labelled oxine sulphate, tropolone or 2-mercaptopyridine-N-oxide (MPO). Under in vitro and in vivo conditions, platelet functions were evaluated by measuring their aggregability, survival, recovery and early distribution. High labelling efficiency was achieved in saline labelling, whereas with plasma labelling, it was necessary to concentrate the platelet-rich plasma to 4.8 x 10(6) platelets/microliters. The aggregation of platelets labelled in plasma or saline was compared with that of controls; platelets labelled in saline showed lower aggregability in 2 microM ADP but not in 5 microM ADP nor with collagen. No significant differences in platelet survival and recovery were noted between platelets labelled in plasma and those labelled in saline. Our results indicate that partial loss of ADP aggregability in vitro does not influence the in vivo viability of platelets labelled in saline. Scintigraphic studies showed that platelets labelled in a saline medium were temporarily sequestrated in the liver but not in the spleen or heart. Thus, platelet labelling in saline does not affect platelet function adversely, but platelets labelled in plasma are more desirable for assessing the early distribution of platelets in the reticuloendothelial system.
使用铟 - 111标记的硫酸氧喹啉、托酚酮或2 - 巯基吡啶 - N - 氧化物（MPO）在有无血浆的情况下对人血小板进行标记。在体外和体内条件下，通过测量血小板的聚集性、存活率、回收率和早期分布来评估血小板功能。在盐水标记中实现了高标记效率，而在血浆标记中，有必要将富含血小板的血浆浓缩至4.8×10⁶个血小板/微升。将在血浆或盐水中标记的血小板的聚集性与对照进行比较；在盐水中标记的血小板在2微摩尔ADP存在下显示出较低的聚集性，但在5微摩尔ADP或胶原存在下则不然。在血浆中标记的血小板和在盐水中标记的血小板之间，在血小板存活率和回收率方面未观察到显著差异。我们的结果表明，体外ADP聚集性的部分丧失并不影响在盐水中标记的血小板的体内活力。闪烁扫描研究表明，在盐溶液中标记的血小板暂时滞留在肝脏中，而不在脾脏或心脏中。因此，在盐水中标记血小板不会对血小板功能产生不利影响，但在血浆中标记的血小板对于评估血小板在网状内皮系统中的早期分布更理想。
