Horman I, Brambilla E
Int J Vitam Nutr Res. 1982;52(2):134-42.
1H-n. m. r. and t. l. c. measurements show that ortho-diphenols induced little or no chemical change in thiamine when co-dissolved in aqueous solution at pH 7.8. Thiamine determinations on the same solutions by the classical thiochrome method are critically susceptible to the amount of dissolved oxygen. An oxygen saturated equimolar solution of thiamine and pyrocatechol (0.03 mM), after 24 hours at pH 7.8 and at 37 degree, gives almost no thiochrome fluorescence response unless the solution is first degassed to remove all traces of oxygen. It is suggested that earlier literature reports of the pronounced anti thiamine effect of o-diphenols were erroneously based on this apparent disappearance of thiamine when oxygen is not excluded.
1H-核磁共振和薄层层析测量结果表明，当在pH 7.8的水溶液中共溶解时，邻二酚对硫胺素几乎没有引起化学变化。用经典硫色素法对相同溶液进行硫胺素测定时，对溶解氧的量极为敏感。硫胺素和邻苯二酚（0.03 mM）的氧饱和等摩尔溶液，在pH 7.8和37℃下放置24小时后，几乎没有硫色素荧光反应，除非该溶液先进行脱气以除去所有痕量的氧。有人认为，早期文献中关于邻二酚对硫胺素具有显著抗硫胺素作用的报道，错误地基于在不排除氧气时硫胺素的这种明显消失。
