异丙肾上腺素诱导心肌坏死后心肌铜变化的时间曲线。

A temporal profile of changes in myocardial copper after isoproterenol induced cardiac necrosis.

作者信息

Mathew B M, Ahmad M, Kumar S, Seth T D, Mahdi S Q, Jamil S A

出版信息

Jpn Circ J. 1978 Jun;42(6):695-9. doi: 10.1253/jcj.42.695.

DOI:10.1253/jcj.42.695

Abstract

The changes in myocardial copper were studied over a period of five days after producing myocardial necrosis in albino rats by subcutaneous injection of isoproterenol. Two different doses of isoproterenol (85 mg/kg & 42.5 mg/kg) were used to study the effect of variation in the extent of myocardial damage on the behaviour of myocardial copper. It was interesting to note that there was mainly an increase in myocardial copper with the lower dose of isoproterenol whereas there was mainly a fall in myocardial copper with the higher dose of isoproterenol. It has been hypothesized that this difference in behaviour of myocardial copper with respect to the different doses of isoproterenol is related to the extent of the myocardial tissue which escapes injury. Confirmation of myocardial necrosis was done by ECG, enzymes (SGOT, SGPT & LDH) and histology. Estimation of myocardial copper was done by atomic absorption spectrophotometry (Perkin Elmer 303).

摘要

通过皮下注射异丙肾上腺素在白化大鼠中造成心肌坏死后，对其心肌铜含量的变化进行了为期五天的研究。使用两种不同剂量的异丙肾上腺素（85毫克/千克和42.5毫克/千克）来研究心肌损伤程度的变化对心肌铜行为的影响。值得注意的是，较低剂量的异丙肾上腺素主要导致心肌铜含量增加，而较高剂量的异丙肾上腺素主要导致心肌铜含量下降。据推测，心肌铜在不同剂量异丙肾上腺素作用下的这种行为差异与未受损伤的心肌组织范围有关。通过心电图、酶（谷草转氨酶、谷丙转氨酶和乳酸脱氢酶）和组织学检查来确认心肌坏死。通过原子吸收分光光度法（珀金埃尔默303）测定心肌铜含量。