Tsanev R G, Djondjurov L P
J Cell Biol. 1982 Sep;94(3):662-6. doi: 10.1083/jcb.94.3.662.
Mouse erythroleukemia cell nuclei obtained by three different methods were spread for electron microscopy under low ionic conditions. It was found that this procedure allows the observation of free large ribonucleoprotein (RNP) complexes released from the nuclei during the centrifugation. The morphology of these complexes was readily affected by the conditions of cell treatment and spreading. Two extreme forms of free nuclear RNP structures were obtained, both consisting of spherical particles with diameters of approximately 17-20 nm. The first type was of loosened complexes of irregularly assembled particles interconnected with RNA fibrils. The second represented tightly packed particles forming mostly branched structures. The latter structures appeared to be closer to the native form of the nuclear RNP particles, differing from polyribosomes by their characteristic branching and stability in EDTA solutions.
通过三种不同方法获得的小鼠红白血病细胞核在低离子条件下铺展用于电子显微镜观察。结果发现，该程序能够观察到在离心过程中从细胞核释放出的游离大核糖核蛋白（RNP）复合物。这些复合物的形态很容易受到细胞处理和铺展条件的影响。获得了两种极端形式的游离核RNP结构，两者均由直径约为17 - 20 nm的球形颗粒组成。第一种类型是由不规则组装的颗粒与RNA纤维相互连接形成的松散复合物。第二种则是紧密堆积的颗粒，主要形成分支结构。后一种结构似乎更接近核RNP颗粒的天然形式，与多核糖体的区别在于其特征性的分支以及在EDTA溶液中的稳定性。
