Deutsch C, Price M
J Cell Physiol. 1982 Oct;113(1):73-9. doi: 10.1002/jcp.1041130113.
The transmembrane electrical potential has been measured across human peripheral blood lymphocytes under culture conditions using equilibrium distributions of the lipophilic cation 3H-tetraphenylphosphonium (TPP). The TPP equilibrates to a steady-state level that gives calculated voltages of -80 mV for unstimulated lymphocytes. This value of the potential is constant during the first few hours of succinyl concanavalin A stimulation. When the transmembrane electrical potential is lowered by isotonic replacement of Na with K, this neither by itself stimulates proliferation nor does it inhibit mitogen-stimulated proliferation. Lymphocytes with similar membrane potentials, such as those incubated in normal-Na MEM and low-Na-mannitol MEM, exhibit drastically different proliferative responses to mitogen stimulation (Deutsch et al., 1981). These results show that isotonic replacement of K for Na prevents low Na inhibition of DNA synthesis and that at least during the first 2.5 hours of lymphocyte activation transmembrane electrical potential per se does not play a significant role in the activation process.
利用亲脂性阳离子3H-四苯基鏻（TPP）的平衡分布，在培养条件下测量了人外周血淋巴细胞的跨膜电势。TPP平衡到一个稳态水平，对于未刺激的淋巴细胞，计算出的电压为-80 mV。在琥珀酰伴刀豆球蛋白A刺激的最初几个小时内，该电势值是恒定的。当用K等渗替代Na使跨膜电势降低时，这本身既不刺激增殖，也不抑制有丝分裂原刺激的增殖。具有相似膜电势的淋巴细胞，如在正常Na的MEM和低Na-甘露醇MEM中孵育的淋巴细胞，对有丝分裂原刺激表现出截然不同的增殖反应（Deutsch等人，1981年）。这些结果表明，用K等渗替代Na可防止低Na对DNA合成的抑制，并且至少在淋巴细胞激活的最初2.5小时内，跨膜电势本身在激活过程中不发挥重要作用。
