采用高效液相色谱法同时测定人和犬血浆及尿液中丹曲林及其代谢产物5-羟基丹曲林和硝基还原乙酰化丹曲林（F 490）。

Simultaneous determination of dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F 490), in plasma and urine of man and dog by high-performance liquid chromatography.

作者信息

Wuis E W, Grutters A C, Vree T B, van der Kleyn E

出版信息

J Chromatogr. 1982 Sep 10;231(2):401-9. doi: 10.1016/s0378-4347(00)81864-2.

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of the skeletal muscle relaxant dantrolene and its metabolites, 5-hydroxydantrolene and nitro-reduced acetylated dantrolene (F-490), in plasma and urine of man and dog. The substances are detected spectrophotometrically at 375 nm. The detection limits are 0.02 mg/l. A preliminary extraction step into a choloroform-butanol mixture is required for the plasma samples. The method is suitable for pharmacokinetic studies of dantrolene.

摘要

描述了一种反相高效液相色谱法，用于同时测定人和犬血浆及尿液中骨骼肌松弛剂丹曲林及其代谢物5-羟基丹曲林和硝基还原乙酰化丹曲林（F-490）。这些物质在375nm处用分光光度法进行检测。检测限为0.02mg/l。血浆样品需要先萃取到氯仿-丁醇混合物中。该方法适用于丹曲林的药代动力学研究。