Liquid chromatography in pharmaceutical analysis VII: determination of dantrolene sodium in biological fluids.

Dantrolene sodium can be determined in plasma and urine samples by high-pressure liquid chromatography without interference from its two major metabolites, the hydroxy and acetamido compounds. The minimum detectability of the drug using this procedure is 8 ng. The complete assay including extraction, evaporation, and separation steps can be performed in approximately 70 min with an accuracy of 1--3%.