Ferlito S, La Spina E, Cultrera E, Puleo F, Carrà G
Arch Sci Med (Torino). 1982 Apr-Jun;139(2):175-81.
The Authors investigated on the main lipidemic parameters in vascular diseases after acute administration of glucuronyl-glucosamine-glycan sulfate (3GS). The study was carried out in 48 subjects, aged 33 to 78, of whom 38 males and 10 females so divided: 6 metabolically healthy controls (N), 10 with vascular cerebral disease (VCD), 15 with coronary heart disease, 17 with peripheral vascular disease (PVD). Each subject, fasting and without drug treatment at least since 14 hours, after venous blood withdrawal, was given endovenous rapid bolus of 570 lipasemic units of glucuronyl-glucosamine-glycan sulfate (3GS); after 10', 20' and 30' other blood samples were taken; plasma NEFA (colorimetric method) triglycerides (TG), total cholesterol (TC) and HDL-cholesterol (C-HDL) (enzyme method) were assayed; C-HDL/TC ratio was calculated and LDL-cholesterol value (C-LDL) according to the Friedwald-Fredrickson's formula was obtained. In controls NEFA increase after 20' (+ 57%) and triglycerides drop (-49%) were detected; TC significant fall (-13%, P less than 0,05) after 20' was found, preceded by both C-HDL marked increase and C-HDL/TC ratio, and followed (30') by C-LDL slight rise. In the 3 groups of vascular patients a striking NEFA increase (respectively + 50%, + 43%, + 26%) was found; in subjects with PVD/TG blunted drop was observed, whereas in VCD and in CHD TG percentage fall -41 and -44%) did not differ from that one of controls. Evident remarked in control, drop of total cholesterolemia, lacked in the 3 groups of vascular diseases; in patients with VCD and PVD C-HDL and C-HDL/TC ratio increasing profile was like normal, while in CHD both progressive reduction of C-HDL (-19% after 30') and C-HDL/TC ratio with C-LDL increase were appreciated. On the basis of their results the Authors believed that in PVD a striking deficit of total plasma lipoprotein lipase (LPL), already in vitro documented, may occur, whereas LPL resulted normal in VCD and in CHD; on the contrary, in CHD an abnormally cholesterol-apo-VLDL affinity, like metabolic atherogenic derangement, was found; in the 3 groups of vascular diseases slow peripheral cholesterol removal was detectable.
作者研究了急性给予硫酸葡糖醛酸 - 葡糖胺聚糖（3GS）后血管疾病中的主要血脂参数。该研究在48名年龄在33至78岁的受试者中进行，其中38名男性和10名女性，分为：6名代谢健康对照（N），10名患有脑血管疾病（VCD），15名患有冠心病，17名患有外周血管疾病（PVD）。每位受试者至少禁食且停药14小时后，静脉采血，然后静脉快速推注570脂解单位的硫酸葡糖醛酸 - 葡糖胺聚糖（3GS）；10分钟、20分钟和30分钟后采集其他血样；测定血浆非酯化脂肪酸（比色法）、甘油三酯（TG）、总胆固醇（TC）和高密度脂蛋白胆固醇（C - HDL）（酶法）；计算C - HDL/TC比值，并根据Friedwald - Fredrickson公式获得低密度脂蛋白胆固醇值（C - LDL）。在对照组中，20分钟后检测到非酯化脂肪酸增加（+57%）和甘油三酯下降（-49%）；发现20分钟后总胆固醇显著下降（-13%，P＜0.05），之前高密度脂蛋白显著增加和C - HDL/TC比值增加，随后（30分钟）低密度脂蛋白略有上升。在3组血管疾病患者中，发现非酯化脂肪酸显著增加（分别为+50%、+43%、+26%）；在外周血管疾病患者中观察到甘油三酯下降不明显，而在脑血管疾病和冠心病患者中甘油三酯下降百分比（-41%和-44%）与对照组无差异。对照组中明显的总胆固醇血症下降在3组血管疾病中未出现；在脑血管疾病和外周血管疾病患者中，高密度脂蛋白和C - HDL/TC比值的增加情况与正常情况相似，而在冠心病患者中，高密度脂蛋白（30分钟后-19%）和C - HDL/TC比值均逐渐降低，同时低密度脂蛋白增加。基于他们的结果，作者认为在外周血管疾病中可能存在总血浆脂蛋白脂肪酶（LPL）明显缺乏，这在体外已有记录，而在脑血管疾病和冠心病中LPL结果正常；相反，在冠心病中发现胆固醇与载脂蛋白 - 极低密度脂蛋白亲和力异常，类似于代谢性动脉粥样硬化紊乱；在3组血管疾病中可检测到外周胆固醇清除缓慢。
