Paolini M, Bauer C, Corsi C, Bronzetti G, Del Carratore R, Nieri R
Boll Soc Ital Biol Sper. 1982 Sep 30;58(18):1164-8.
A short diethyl ether anesthesia before the three injections to induce xenobiotic metabolizing monooxygenase in mice for the preparation of liver S-9 fraction used in mutagenesis test in vitro does not negatively alter the biochemical properties of the activating system and gives unchanged genetic results. Instead, the use of more prolonged ether anesthesia also at the day of the sacrifice, results in less active and less stable enzymes in the S-9 fraction, which can lead to altered genetic results.
在三次注射前进行短时间的乙醚麻醉，以诱导小鼠体内的外源物代谢单加氧酶，用于制备体外诱变试验中使用的肝脏S-9组分，这不会对激活系统的生化特性产生负面影响，并且遗传结果不变。相反，在处死当天也使用更长时间的乙醚麻醉，会导致S-9组分中的酶活性降低且稳定性变差，这可能会导致遗传结果改变。
