Burger M M, Tao T W, Finne J, Prieels J P
Biosci Rep. 1982 Aug;2(8):597-9. doi: 10.1007/BF01314221.
In an effort to assess the effect of surface carbohydrates upon the metastasizing properties of tumor cells, lectin-resistant mouse melanoma cells were selected. Wheat-germ-agglutinin-resistant lines displayed mainly decreased metastasis properties as well as well-defined alterations in surface carbohydrates: in a glycopeptide with four side chains, two of them were missing their terminal sialic acid residues while two fucoses were newly attached to the oligosaccharide. The enzymatic defect could be pinpointed to an over-60-fold increase in fucosyltransferase, while the sialyltransferase did not decrease significantly. Revertants were again selected with lectins and their fucosyltransferase activities returned to normal values again. The metastasizing potential of the revertants was not yet assessed carefully but a return of some of the metastasizing potential was noted.
为了评估表面碳水化合物对肿瘤细胞转移特性的影响，我们选择了抗凝集素的小鼠黑色素瘤细胞。抗麦胚凝集素细胞系主要表现出转移特性降低以及表面碳水化合物有明确的改变：在一个带有四条侧链的糖肽中，其中两条缺失了末端唾液酸残基，而两条岩藻糖新连接到了寡糖上。酶缺陷可以精确到岩藻糖基转移酶增加了60多倍，而唾液酸基转移酶没有显著降低。再次用凝集素筛选回复体，其岩藻糖基转移酶活性又恢复到正常值。回复体的转移潜力尚未仔细评估，但注意到其转移潜力有所恢复。
